| 초록 |
Although culture-independent studies have shown the abundance of the order Burkholderiales in deep-sea environment, deep-sea members of the order have been rarely cultured and characterized. In the present study, a Gram-stain-negative, rod-shaped, aerobic and motile by a single flagellum, designated SAORIC-580T, was isolated from deep seawater (4,000 m) in the Northwest Pacific Ocean. The 16S rRNA gene sequence analysis showed that the strain is affiliated to the genus Limnobacter and most closely related to L. thiooxidans CS-K2T sharing 99.9% sequence similarity. Whole genome sequence of strain SAORIC-580T was 3.3 Mbp of genome size with 52.5 mol% of the DNA G + C content. The SAORIC-580T genome shared less than 79.4–85.7% of the average nucleotide identity and 19.9–29.5% of digital DNA-DNA hybridization values to the Limnobacter genomes, indicating that the novel strain represents a novel species in the genus. The isoprenoid quinone of strain SAORIC-580T was ubiquione-10. The major cellular fatty acids were summed feature 8 (C18:1 ω7c), C18:1 ω7c 11-methyl and C16:0. The major polar lipids constituted phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol. On the basis of taxonomic data obtained in this study, it was concluded that strain SAORIC-580T represented a novel species of the genus Limnobacter, for which the name Limnobacter profundi sp. nov. is proposed. The type strain is SAORIC-580T (= KACC 21440T = NBRC 114111T). |
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